GALLY International Biomedical Research & Consulting LLC was
founded by Gjumrakch Aliev, M.D., Ph.D. and is operated by the
GALLY International Management Team.
GALLY International Biomedical Research & Consulting LLC provides
expertise in light and electron microscopy to clients in both
academia and commercial industry worldwide. We will provide
assistance with the experimental design, microscopic analysis, and
manuscript and grant writing for both biological and non-biological
studies for the BS, MS as well as MD&PhD program and postdoctoral
students and scientist at any level and any other organization
looking for modern ultrastructural analysis.
Dr. Aliev has extensive expertise in all of the various aspects
of microscopic analysis such as experimental design, data analysis,
and manuscript/grant writing for both biological and non-biological
studies. With many years of experience in LM, EM, 2-photon
microscopy, Atomic Force and Confocal Microscopy he has produced
pioneering work in different areas of EM, including new methods of
EM techniques. These techniques include cytological in situ
hybridization at the light and electron microscopic levels using
non-isotopic colloidal gold probes, peroxidase-anti-peroxidase
(PAP), and pre- and post- embedding single double and triple
immunogold cytochemistry and quantification. His methods have been
included in studies published in a number of highly recognized
journals (for details, please see the list of publications in the
media portion of our site and pubmed search: aliev g).
Professor Aliev also continues an active research program. One of
his latest research interests focuses on investigating the
interaction of nanoparticles with tissues and cells. He has
recently developed ways to determine the biological effects of
nanoparticles in vivo and in vitro by utilizing animal models of
human diseases. The goal of this study is to not only elucidate the
pathogenic mechanisms underlying the nanoparticles' effects, but to
also discover potential new drug development strategies. For
example, ongoing several projects supervising by Professor Aliev
(Strategies for Drug Development and Delivering to Central Nervous
System) has so far showed promising results regarding the specific
delivery of drugs to posttraumatic, tumor and animal model that
mimics human neurodegeneration. In another of Professor Aliev in
vivo studies, he had found that nanoparticles are able to cross the
blood brain barrier (BBB), which has been the biggest impediment in
delivering drugs to patients with Posttraumatic Brain Injury as
well as Alzheimer disease. GALLY International’s teams of
professionals are using classical biochemistry, cell biology, and
morphology techniques in conjunction with more modern methods, such
as SEM/TEM SED X-ray elemental analysis and NMR studies of protein
structure and dynamics, macromolecular interactions, mechanistic
enzymology, and computational analyses and modeling, in order to
determine the exact nature of the relationship between the
nanoparticles and the underlying tissue.
Please visit our websites for more details on these publications
Techniques Utilized by the GALLY
International Biomedical Research & Consulting:
- Pre-Embedding Immunoelectron Microscopy
Peroxidase-anti-Peroxidase (PAP) and Avidin-Biotin (ABC)
- Pre- and Post-Embedding Immunoelelctron Microscopy Single,
Double and Triple Gold Labeling techniques.
- Light Microscopy Single and Double Immunofluorescent Labeling
- Light and EM Immunohistochemical techniques.
- Laser Confocal Microscopy techniques.
- Different modification of Light Microscopy (LM) and
Transmission Electron Microscopy (TEM) techniques.
- Scanning Electron Microscopy Native Specimens techniques.
- TEM Ruthenium Red, Horseradish Peroxidase and other Tracer
- LM, SEM and TEM Autoradiographic methods.
- SEM and TEM frozen-Fracture techniques.
- SEM intraorgan and Intracellular Structure techniques.
- SEM and TEM Detergent Extraction techniques.
- LM, SEM and TEM Silver staining methods.
- SEM plastic replica and corrosion casts preparation.
- Light and EM Morphometric Measurements.
- Quantification of Gold Particles and Image Analysis.
- In Situ Hybridization at Light and EM levels (non-isotopic
- Small and Large laboratory Animal Surgery, including Vascular,
Nervous as well as other system Injury techniques.
- Tissue Culture Techniques.
- Perfusion Fixation.
- SEM and TEM organic and non-organic material investigation
including data interpretation.
- Two and three dimensional (TEM and SEM, respectively) low
vacuum techniques including natural and organic material
investigation and interpretation.
- Atomic Force Microscopic techniques.
In addition, the highly trained staff of professionals at GALLY
International Biomedical Research & Consulting LLC are able to
assist in experimental modeling, perfusion fixation, and tissue
Examples of work performed by the
GALLY International Biomedical Research & Consulting LLC:
- Perfusion Fixation: The trained technical staff at GALLY
International can either assist or perform the perfusion fixation
of large and/or small laboratory animals as well as cell culture
or cell suspensions. Fresh solutions will be prepared for each
perfusion. The company also provides vibratome block sectioning
for future immuno or routine microscopic investigations.
- Routine Light Microscope semithin sections (LM and EM):
Samples, including fresh tissue specimens, cultured cells on
substrate, subcellular fractions (for purity determinations) as
well as plastic or other organic and non-organic materials can be
routinely processed (paraformaldehyde/glutaraldehyde-osmium fixed,
dehydrated plastic embedded) and thick or ultrathin sections
- Immunolocalization: Studies at both the light and EM level can
be performed using pre- and post-embedding single, double, or
triple immunogold and/or peroxidase-anti-peroxidase (PAP) or other
tracers to visualize target molecules. Examples of
Immunolocalization studies using colloidal gold and PAP techniques
at the light and EM levels can be found in our recent
- Cytological Localization of in situ hybridization markers for
DNA: Studies at both the light and EM levels can be performed
using PAP and colloidal gold (non-direct, non-isotopic) probes to
visualize the cytological localization of DNA markers at the
cellular and subcellular levels. We have found that cytological
localization of in situ hybridization markers for wild and deleted
mitochondrial DNA provide more correct information about the
mitochondria DNA deletions than that other techniques, including
PCR. (For details on in situ hybridization for mitochondrial DNA
markers at the light and EM levels please see: J Neurosci
21:3017-3023, 2001; Aliev G, et al., Brain mitochondria as a
primary target in the development of treatment strategies for
Alzheimer disease. Int J Biochem Cell Biol. 2009
Oct;41(10):1989-2004; Aliev G. et al., Atherosclerotic lesions and
mitochondria DNA deletions in brain microvessels: implication in
the pathogenesis of Alzheimer's disease. Vasc Health Risk Manag.
2008;4(3):721-30). If you have any DNA probes we will be very
happy to employ our expertise in different tissue and /or cell
culture or cell suspension systems.
- Rapid freezing of tissue samples can be used for
Immunolocalization of freeze-substitution.
- GALLY International Biomedical Research & Consulting can employ
the techniques of either negative staining or rotary metal
shadowing of individual proteins to allow high resolution imaging
of the shapes and dimensions of individual protein molecules or
- Using the automated computerized systems, high quality
immunofluorescent and PAP stains can be obtained using different
antibodies for different tissue or cell culture systems.
- Three dimensional structural organizations of the cells,
tissue, organs as well as intracellular compartments can be
performed by using Scanning Electron Microscopy. Our highly
trained staff can perform tissue processing, critical point
drying; sputter coating, microscope operation and imaging of
cells, tissues, and other organic or non-organic materials.
- Data Interpretation: The GALLY International Biomedical
Research & Consulting LLC also offers data analysis and
interpretation along and with assistance in manuscript and grant
writing. The prices for these services are negotiated with the
client. The GALLY International Biomedical Research & Consulting
LLC provides comprehensive training courses on transmission,
scanning and light microscopy procedures for researchers in both
academia and industry. In addition, we will provide other
supplemental services including electronic data transfer.