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GALLY International Biomedical Research & Consulting LLC Electron Microscopy Expertise Services for Biological and Non-Biological Materials:

GALLY International Biomedical Research & Consulting LLC provides expertise in light and electron microscopy to clients in both academia and commercial industry worldwide. We will provide assistance with the experimental design, microscopic analysis, and manuscript and grant writing for both biological and non-biological studies for the BS, MS as well as MD&PhD program and postdoctoral students and scientist at any level and any other organization looking for modern ultrastructural analysis.

Dr. Aliev has extensive expertise in all of the various aspects of microscopic analysis such as experimental design, data analysis, and manuscript/grant writing for both biological and non-biological studies. With many years of experience in LM, EM, 2-photon microscopy, Atomic Force and Confocal Microscopy he has produced pioneering work in different areas of EM, including new methods of EM techniques. These techniques include cytological in situ hybridization at the light and electron microscopic levels using non-isotopic colloidal gold probes, peroxidase-anti-peroxidase (PAP), and pre- and post- embedding single double and triple immunogold cytochemistry and quantification. His methods have been included in studies published in a number of highly recognized journals (for details, please see the list of publications in the media portion of our site and pubmed search: aliev g).

GALLY International’s management team also continues an active research program. One of it’s latest research interests focuses on investigating the interaction of nanoparticles with tissues and cells. Dr. Aliev has recently developed ways to determine the biological effects of nanoparticles in vivo and in vitro by utilizing animal models of human diseases. The goal of this study is to not only elucidate the pathogenic mechanisms underlying the nanoparticles' effects, but to also discover potential new drug development strategies. For example, ongoing several projects supervising by Professor Aliev (Strategies for Drug Development and Delivering to Central Nervous System) has so far showed promising results regarding the specific delivery of drugs to posttraumatic, tumor and animal model that mimics human neurodegeneration. In another of Professor Aliev in vivo studies, he had found that nanoparticles are able to cross the blood brain barrier (BBB), which has been the biggest impediment in delivering drugs to patients with Posttraumatic Brain Injury as well as Alzheimer disease. GALLY International’s teams of professionals are using classical biochemistry, cell biology, and morphology techniques in conjunction with more modern methods, such as SEM/TEM SED X-ray elemental analysis and NMR studies of protein structure and dynamics, macromolecular interactions, mechanistic enzymology, and computational analyses and modeling, in order to determine the exact nature of the relationship between the nanoparticles and the underlying tissue.

Techniques Utilized by the GALLY International:
  1. Pre-Embedding Immunoelectron Microscopy Peroxidase-anti-Peroxidase (PAP) and Avidin-Biotin (ABC) Cytochemistry techniques.
  2. Pre- and Post-Embedding Immunoelelctron Microscopy Single, Double and Triple Gold Labeling techniques.
  3. Light Microscopy Single and Double Immunofluorescent Labeling methods.
  4. Light and EM Immunohistochemical techniques.
  5. Laser Confocal Microscopy techniques.
  6. Different modification of Light Microscopy (LM) and Transmission Electron Microscopy (TEM) techniques.
  7. Scanning Electron Microscopy Native Specimens techniques.
  8. TEM Ruthenium Red, Horseradish Peroxidase and other Tracer techniques.
  9. LM, SEM and TEM Autoradiographic methods.
  10. SEM and TEM frozen-Fracture techniques.
  11. SEM intraorgan and Intracellular Structure techniques.
  12. SEM and TEM Detergent Extraction techniques.
  13. LM, SEM and TEM Silver staining methods.
  14. SEM plastic replica and corrosion casts preparation.
  15. Light and EM Morphometric Measurements.
  16. Quantification of Gold Particles and Image Analysis.
  17. In Situ Hybridization at Light and EM levels (non-isotopic techniques).
  18. Small and Large laboratory Animal Surgery, including Vascular, Nervous as well as other system Injury techniques.
  19. Tissue Culture Techniques.
  20. Perfusion Fixation.
  21. SEM and TEM organic and non-organic material investigation including data interpretation.
  22. Two and three dimensional (TEM and SEM, respectively) low vacuum techniques including natural and organic material investigation and interpretation.
  23. Atomic Force Microscopic techniques.

In addition, the highly trained staff of professionals at GALLY International Biomedical Research & Consulting LLC are able to assist in experimental modeling, perfusion fixation, and tissue culture.

Examples of work performed by the GALLY International:
  • Perfusion Fixation: The trained technical staff at GALLY International can either assist or perform the perfusion fixation of large and/or small laboratory animals as well as cell culture or cell suspensions. Fresh solutions will be prepared for each perfusion. The company also provides vibratome block sectioning for future immuno or routine microscopic investigations.
  • Routine Light Microscope semithin sections (LM and EM): Samples, including fresh tissue specimens, cultured cells on substrate, subcellular fractions (for purity determinations) as well as plastic or other organic and non-organic materials can be routinely processed (paraformaldehyde/glutaraldehyde-osmium fixed, dehydrated plastic embedded) and thick or ultrathin sections obtained.
  • Immunolocalization: Studies at both the light and EM level can be performed using pre- and post-embedding single, double, or triple immunogold and/or peroxidase-anti-peroxidase (PAP) or other tracers to visualize target molecules. Examples of Immunolocalization studies using colloidal gold and PAP techniques at the light and EM levels can be found in our recent publications.
  • Cytological Localization of in situ hybridization markers for DNA: Studies at both the light and EM levels can be performed using PAP and colloidal gold (non-direct, non-isotopic) probes to visualize the cytological localization of DNA markers at the cellular and subcellular levels. We have found that cytological localization of in situ hybridization markers for wild and deleted mitochondrial DNA provide more correct information about the mitochondria DNA deletions than that other techniques, including PCR. (For details on in situ hybridization for mitochondrial DNA markers at the light and EM levels please see: J Neurosci 21:3017-3023, 2001; Aliev G, et al., Brain mitochondria as a primary target in the development of treatment strategies for Alzheimer disease. Int J Biochem Cell Biol. 2009 Oct;41(10):1989-2004; Aliev G. et al., Atherosclerotic lesions and mitochondria DNA deletions in brain microvessels: implication in the pathogenesis of Alzheimer's disease. Vasc Health Risk Manag. 2008;4(3):721-30). If you have any DNA probes we will be very happy to employ our expertise in different tissue and /or cell culture or cell suspension systems.
  • Rapid freezing of tissue samples can be used for Immunolocalization of freeze-substitution.
  • GALLY International can employ the techniques of either negative staining or rotary metal shadowing of individual proteins to allow high resolution imaging of the shapes and dimensions of individual protein molecules or complexes.
  • Using the automated computerized systems, high quality immunofluorescent and PAP stains can be obtained using different antibodies for different tissue or cell culture systems.
  • Three dimensional structural organizations of the cells, tissue, organs as well as intracellular compartments can be performed by using Scanning Electron Microscopy. Our highly trained staff can perform tissue processing, critical point drying; sputter coating, microscope operation and imaging of cells, tissues, and other organic or non-organic materials.
  • Data Interpretation: The GALLY International Biomedical Research & Consulting LLC also offers data analysis and interpretation along and with assistance in manuscript and grant writing. The prices for these services are negotiated with the client. GALLY International provides comprehensive training courses on transmission, scanning and light microscopy procedures for researchers in both academia and industry. In addition, we will provide other supplemental services including electronic data transfer.